we try adding half and double the amount of bead solution and did not find much difference in the final Ct. One thing that really helps us was to increase Ct was the incubation of 10 min @ 60 C with Proteinase K.

We have not tested the addition of mucolytics to the extraction, but Alexander James Phillips suggested several reagents…Read more

we have started diagnosis at my institution (AGROSAVIA, Colombia)  two weeks ago and we have been running in parallel both the column extraction kit and your protocol for around 20 samples.  We have noticed that the RNaseP gene amplifies at the same CT (human RNA extraction internal control), however, the RdRP gene (SARS-Cov2) a…Read more

thank you so much for providing this open protocols for everybody, I am currently involved in SARS-CoV2 detection in Colombia, and we are currently trying to start setting up a commercial kit vs the BOMB.bio protocol, we have some questions about the preparation of the GITC buffer, specifically what options in terms of reagents we have…Read more