I tried a protocol that incorporated all your suggestions (proteinase K digestion, isopropanol in binding buffer, chaotropic salt and/or EtOH in wash buffers) and got a much better yield.

It was still lower when extracting from plasma than when extracting from pure water, but that is probably to be expected considering the protein…Read more

Thank you very much for your answer! I really appreciate your inputs 🙂

Can you explain me why it is not a functional DNA extraction method? The “protocol” (including buffer recipes) was supplied together with the silica particles from the manufacturer. It is intended for purification of viral DNA from plasma, but I thought it might work…Read more

I am trying to purify DNA from plasma samples, without the use of a commercial kit, as i want to have insight into the buffers I use. I use commercial silica beads (MagPrep silica particles from Merck) together with the following buffers:

Binding buffer: 5 M GTC, 1% Triton X-100, 10 mM Tris-HCl

Wash buffer: 10 mM Tris-HCl, pH…Read more