bomb.bio | Cor Breukel | Activity https://bomb.bio/members/corb/activity/ Activity feed for Cor Breukel. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 7700ca18af0a3b213ef5e21dc644d8c0 Cor Breukel replied to the topic BOMB protocol #5.1 plasmid DNA extraction from E.coli in the forum Plasmid Extraction https://bomb.bio/forums/topic/5-1-plasmid-dna-extraction-from-e-coli/#post-2228 Mon, 01 Apr 2019 09:52:35 +1200 Thanks for the reply !

Best,

Cor

]]> 70ca3ab0b31535f592622451f078875f Cor Breukel replied to the topic BOMB protocol #5.1 plasmid DNA extraction from E.coli in the forum Plasmid Extraction https://bomb.bio/forums/topic/5-1-plasmid-dna-extraction-from-e-coli/#post-2193 Tue, 26 Mar 2019 15:27:06 +1200 Hi,

Thanks for the provided protocols, I appreciate the effort ! I made my own beads and succesfully preped the first mini preps. I did use the buffers I had left over from a ROCHE kit since they are nearly the same.

 

I have a few questions:

In step 8 you state to mix first with ethanol for 5 minutes and than add the beads. I did  add…Read more

]]> 18fe7202a4fab44bebc21e112c722a38 Cor Breukel became a registered member https://bomb.bio/activity/p/229/ Tue, 26 Mar 2019 15:17:15 +1200 0