bomb.bio | Emilly | Activity https://bomb.bio/members/emijarina/activity/ Activity feed for Emilly. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 83117ab7b9dac326b71e660df9624d8f Emilly replied to the topic BOMB protocol #7.1 gDNA extraction in the forum gDNA https://bomb.bio/forums/topic/bomb-protocol-7-1-gdna-extraction/page/2/#post-9722 Sun, 20 Mar 2022 20:57:57 +1200 Hi Tim, thanks for your answer!

I will try tissue protocol then. Another question, I was reading some papers and they recommend pH 6 – 7 for silica DNA adsortion, could you explain why your pH lysis buffer is 7,6 – 8?

Best wishes.

]]> c59804eb28ec57df1d4a97b38b194ec5 Emilly replied to the topic BOMB protocol #7.1 gDNA extraction in the forum gDNA https://bomb.bio/forums/topic/bomb-protocol-7-1-gdna-extraction/#post-9712 Thu, 10 Mar 2022 17:22:21 +1200 Hello!

I was reading your protocol of gDNA extraction with beads and a got confused with ratio mentioned 2:3:4. How is it used with blood sample and without TE buffer at beggining? It’s 140ul of blood and 20ul magnetic bead?

Best regards.

]]> 04543d464297b1e500340fa9bc8268da Emilly became a registered member https://bomb.bio/activity/p/5874/ Thu, 10 Mar 2022 17:17:17 +1200 0