bomb.bio | Erik | Activity https://bomb.bio/members/erik21/activity/ Activity feed for Erik. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 812b61805962d49279d01369a69ecc6c Erik started the topic Recovery loss for large volumes - role of wash buffer volume? in the forum Clean-up and size exclusion https://bomb.bio/forums/topic/recovery-loss-for-large-volumes-role-of-wash-buffer-volume/ Mon, 12 Aug 2024 21:39:58 +1200 I’m using this protocol to clean up DNA template after a linearization reaction (removal of buffer & enzyme, rather than size selection). On a small scale, it works quite well with recovery rates around 75-80% out of the box.

However, when I scale it up, I am reaching conditions where my recovery suddenly drops to below 60% while DNA:bead as well…Read more

]]> 9f2854975bc69e2d6c4ec213a71a58f9 Erik became a registered member https://bomb.bio/activity/p/52015/ Wed, 07 Aug 2024 21:41:29 +1200 0