bomb.bio | Jiyi | Activity https://bomb.bio/members/jiyi/activity/ Activity feed for Jiyi. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 bc9d214bc97f990cb8e4f72cc3e29a06 Jiyi replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-8857 Fri, 11 Oct 2019 07:41:01 +1200 Hi Michael,

Thanks for your reply, I repeat my experiment several time as keeping  beads on the magnet at the wash steps. The recovery raised to 20%, which is still low to me.

I am wondering that maybe the key problem is my beads.  I will update my progress if there are some good news.

Best,

Ji

 

]]> 5593f9f81d8a67b65af390d1f3d5151b Jiyi replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-8854 Thu, 10 Oct 2019 08:44:03 +1200 hi everyone,

Is your recovery rate higher than 50%? My recovery is only 10% when I followed the protocol. Only difference is the silica-coated magnetic beads. I purchased the beads from other company. Its concentration is 50mg/ul and the size is 100nm.

The recovery of the EZ DNA methylation MagPrep kit(Zymo) is about 50-70%.

Best,

Ji

]]> 09735695cf544b1c3b142b216650f1f7 Jiyi became a registered member https://bomb.bio/activity/p/3600/ Thu, 10 Oct 2019 08:32:57 +1200 0