bomb.bio | Jennifer Kuehl | Activity https://bomb.bio/members/jvkuehl/activity/ Activity feed for Jennifer Kuehl. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 136d6c9c38fcd554956fe2d46a9f311e Jennifer Kuehl replied to the topic BOMB protocol #7.1 gDNA extraction in the forum gDNA https://bomb.bio/forums/topic/bomb-protocol-7-1-gdna-extraction/#post-9513 Wed, 09 Sep 2020 05:03:59 +1200 Hello

I am also getting very low 260/230 ratios.  I tried up to eight 80% ethanol washes, the ratios  improve with more washing but  they are still low ~.6 for gram positive bacteria and 1.7 for gram negative.  I am considering  a larger volumes of 70% ethanol (600ul) for the wash steps or perhaps a secondary bead clean-up.  Any recommendations?

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