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	<title>bomb.bio | Kylie Drake | Activity</title>
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				<title>Kylie Drake replied to the topic SARS-CoV-2 Purification (Viral Transfer Media) - BOMB extraction protocol in the forum COVID-19</title>
				<link>https://bomb.bio/forums/topic/the-official-sars-cov-2-bomb-extraction-protocol/#post-9309</link>
				<pubDate>Tue, 14 Apr 2020 18:54:44 +1200</pubDate>

									<content:encoded><![CDATA[<p>Thanks Tim.</p>
<p>Alexander &#8211; Tim M is correct. Our focus is on delivering a diagnostic service at present. However, I will take your comments under consideration and we&#8217;ll see what our team can do.</p>
<p>To answer a couple of questions though:</p>
<p>Our validation was compared to the Abbott RealTi&lt;i&gt;m&lt;/i&gt;e SARS-CoV-2 kit and extraction on an Abbott M2000. The&hellip;<span class="activity-read-more" id="activity-read-more-4560"><a href="https://bomb.bio/forums/topic/the-official-sars-cov-2-bomb-extraction-protocol/#post-9309" rel="nofollow ugc">Read more</a></span></p>
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				<title>Kylie Drake started the topic Heat inactivation and BOMB method in the forum COVID-19</title>
				<link>https://bomb.bio/forums/topic/heat-inactivation-and-bomb-method/</link>
				<pubDate>Wed, 08 Apr 2020 23:03:43 +1200</pubDate>

									<content:encoded><![CDATA[<p>Hi all,</p>
<p>All of the initial validation work we did with the BOMB protocol utilised positive patient samples that had been heat inactivated at 56C for 30 minutes.</p>
<p>While there was a slight decrease in the Ct values we achieved with these samples compared to that which was obtained prior to extraction, it was considered negligible. Heat inactivation&hellip;<span class="activity-read-more" id="activity-read-more-4542"><a href="https://bomb.bio/forums/topic/heat-inactivation-and-bomb-method/" rel="nofollow ugc">Read more</a></span></p>
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				<title>Kylie Drake replied to the topic Hillbilly Bead Viral RNA Extraction Protocol - BOMB Community in the forum COVID-19</title>
				<link>https://bomb.bio/forums/topic/hillbilly-bead-viral-rna-extraction-protocol-bomb-community/#post-9265</link>
				<pubDate>Mon, 06 Apr 2020 04:01:28 +1200</pubDate>

									<content:encoded><![CDATA[<p>Emily,</p>
<p>Ideally, collecting straight into GITC will solve the volume problem. But we had to make do with current practice in our laboratory. Who is adding your swabs to VTM? If it isn’t happening at the clinic, consider a move to GITC.</p>
<p>One way to reduce your input volume is to increase the molarity of the GITC lysis buffer. That’s why the COV&hellip;<span class="activity-read-more" id="activity-read-more-4524"><a href="https://bomb.bio/forums/topic/hillbilly-bead-viral-rna-extraction-protocol-bomb-community/#post-9265" rel="nofollow ugc">Read more</a></span></p>
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				<title>Kylie Drake became a registered member</title>
				<link>https://bomb.bio/activity/p/4520/</link>
				<pubDate>Mon, 06 Apr 2020 00:49:43 +1200</pubDate>

				
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