bomb.bio | Erik Lehnsherr | Activity https://bomb.bio/members/magneto/activity/ Activity feed for Erik Lehnsherr. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 a704b6cf98aec076baf684bfa371f69c Erik Lehnsherr replied to the topic Electroelution in the forum New frontiers https://bomb.bio/forums/topic/electroelution/#post-9646 Wed, 17 Nov 2021 13:12:54 +1200 Yes it’s exactly that. I want to use them as a shuttle in a microfluidic channel and stop the beads with a magentic trap on a certain point. I have sources of several kV at my disposal and I intend to USE THEM (ZZZZAAAAAP). To get rid of the other impurities I might apply subsequent capillary electrophoresis.

]]> 3670d838e108ac12078f54016f8dd3cb Erik Lehnsherr started the topic Electroelution in the forum New frontiers https://bomb.bio/forums/topic/electroelution/ Sat, 23 Oct 2021 20:19:50 +1200 For further automation of DNA preparation I am looking for an elegant way of elute the DNA or RNA from beads without any elution buffer or distilled water. Electroelution comes to mind, which can be used for streptovidin/biotin bonding.

Anybody has some experience with that?

]]> 1f054ecc9af2bbfea0b27a8de1e3f405 Erik Lehnsherr started the topic Calculate the amount of beads needed in the forum Reagents https://bomb.bio/forums/topic/calculate-the-amount-of-beads-needed/ Sat, 23 Oct 2021 20:17:22 +1200 Right now I am trying to optimise a protocol for the purification of 300 pmol freshly synthesised DNA < 50 bases.

How can I calculate the right amount of beads needed? Lacking a better method I simply try ever smaller volumes of the MagPrep Silica beads (roughly 100 µm diameter) and determine the recovery rate in the end. I can already tell you…Read more

]]> d0110a90be030a3394b397065784fa3c Erik Lehnsherr changed their profile picture https://bomb.bio/activity/p/5508/ Fri, 22 Oct 2021 09:21:13 +1200 0 c1282f28a4f6e902478654b03e4471df Erik Lehnsherr became a registered member https://bomb.bio/activity/p/5506/ Fri, 22 Oct 2021 07:17:40 +1200 0