bomb.bio | Malgorzata Cebrat | Activity https://bomb.bio/members/mcebrat/activity/ Activity feed for Malgorzata Cebrat. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 89145d879834f3a8f947e01a0b64ff6c Malgorzata Cebrat replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/page/3/#post-18254 Thu, 30 Mar 2023 13:52:12 +1200 An update – I have tested the silica-coated NPs in DNA isolation and they worked very well. So to summarize my experiment: to avoid the desintegration of MNPs during washing with water after the synthesis, I’ve pelleted them with magnet, washed three times in small volume of ethanol (instead of water) and then immediately proceeded to coating with…Read more

]]> 932b39908130f8fd45099301cfc6f20d Malgorzata Cebrat replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/page/3/#post-18253 Wed, 29 Mar 2023 11:12:17 +1200 Hi,

I have successfully synthesized the NPs once but I was never lucky enough to repeat the procedure. I have the same problem as reported in this thread – the NPs during washing with water start to desintegrate, the supernatant is getting brown (or even reddish) and the leftover NPs are becoming less paramagnetic. It seems that, at least in my…Read more

]]> 22d45b5d2276696660d7099d357e92fc Malgorzata Cebrat changed their profile picture https://bomb.bio/activity/p/4349/ Sun, 02 Feb 2020 22:55:25 +1200 0 56b657f405a2f48e389a8c021d30f49a Malgorzata Cebrat replied to the topic BOMB protocol #4.4 BOMB Nucleotide removal using silica-coated MNPs in the forum Clean-up and size exclusion https://bomb.bio/forums/topic/bomb-protocol-4-4-bomb-nucleotide-removal-using-silica-coated-mnps/#post-8919 Sun, 02 Feb 2020 22:53:11 +1200 Hi,

have you ever tried to use the nucleotide removal protocol to cleanup sequencing reactions? There some commercial kits utilizing magnetic beads for this purpose and I was wondering if we could be able to deal with as well in the BOMB system

Best – Gosia

]]> 056f70ec10b25d33295827905bfa33e8 Malgorzata Cebrat replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/page/2/#post-2229 Thu, 04 Apr 2019 10:33:06 +1200 Hi everyone,

I’ve just produced silica-coated MNPs. In my first attempt I’ve tried to do it in the same scale as in the protocol but got poor results – the MNPs  lost their magnetic properties during washes. I’ve scaled down the procedure 4x and it worked very good. I’ve coated approx. 50% of the beads with TEOS with overnight incubation (the…Read more

]]> c53f584250e355c94f32cdc74f702ad5 Malgorzata Cebrat became a registered member https://bomb.bio/activity/p/232/ Wed, 27 Mar 2019 14:37:53 +1200 0