bomb.bio | MCGambetta | Activity https://bomb.bio/members/mcgambetta/activity/ Activity feed for MCGambetta. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 071837c8411a351c79ba117b827449c0 MCGambetta replied to the topic BOMB protocol #5.1 plasmid DNA extraction from E.coli in the forum Plasmid Extraction https://bomb.bio/forums/topic/5-1-plasmid-dna-extraction-from-e-coli/#post-2026 Sun, 28 Oct 2018 15:00:33 +1200 It seems that the Nanodrop over-estimates the DNA concentration of our plasmid minipreps when we use our silica-coated beads. Does anyone have a suggestion? Many thanks

]]> 6c00d0f122982ad5436608bd874c92a3 MCGambetta replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/#post-2025 Sun, 28 Oct 2018 14:56:19 +1200 Thanks for the tips Tomek, now it’s working.

]]> c88b83815121cc53ea52f134a2c17eb2 MCGambetta replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/#post-2018 Wed, 17 Oct 2018 14:26:34 +1200 Right after synthesis, in alkali conditions, the magnetite pelleted on the magnet completely and quickly.

During the washes in H2O the magnetite pelleted more and more slowly, until it hardly pelleted anymore. The supernatant became progressively more brown in color.

We just ordered a better magnet.

Is it normal, though, that the magnetite…Read more

]]> 76add35c920245ecd4e3b61a7f3ddac3 MCGambetta replied to the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/#post-2015 Tue, 16 Oct 2018 16:29:32 +1200 Thanks for this awesome protocol 🙂

We got lovely magnetic beads after step 4 of the protocol…but the beads progressively became less and less magnetic during the MilliQ H2O washes.

I guess the culprit is our MilliQ water – its pH is 5   :-S

Along the lines of what R.B. suggested in reply #1942, could you suggest using buffer (which one?) i…Read more

]]> de1a82282185c5e7badd25084dd43803 MCGambetta became a registered member https://bomb.bio/activity/p/142/ Tue, 16 Oct 2018 16:23:13 +1200 0