bomb.bio | Michael Dunnet | Activity https://bomb.bio/members/michaeldunnet/activity/ Activity feed for Michael Dunnet. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 3172537e8d52a2513d0346c31da3f88e Michael Dunnet replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-8856 Thu, 10 Oct 2019 22:52:25 +1200 Hi Jiyi,

I seem to get equivalent recovery rates with both BOMB and the EZ DNA methylation MagPrep kit. One thing that might be causing the issue is the washing steps. I know the zymo kit asks you to re-suspend the beads upon each wash; however, when I use the BOMB kit I keep the beads on the magnet and just add the ethanol to the tube for around…Read more

]]> 57bcf2113060bd2eb2a5d5247373aef2 Michael Dunnet replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-8759 Mon, 08 Jul 2019 03:05:51 +1200 Hey,

Just a quick question regarding the conversion step of the protocol. I’ve been getting lower conversion efficiencies compared to the previous kit I had been using (the EZ DNA methylation MagPrep kit by Zymo). I’ve noticed that the temperatures in the conversion step are much lower than in the Zymo kit despite everything else been very…Read more

]]> 5dfeeb0002536a3a2c19a2abddba83ed Michael Dunnet became a registered member https://bomb.bio/activity/p/3284/ Mon, 01 Jul 2019 02:25:19 +1200 0