bomb.bio | Mike Axtell | Activity https://bomb.bio/members/mikeaxtell/activity/ Activity feed for Mike Axtell. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 a70fa147252c7ba6b62ff4f2b2951428 Mike Axtell replied to the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/#post-9448 Thu, 09 Jul 2020 19:52:15 +1200 Thanks Phil, I really appreciate your time and advice!

I’m using the plant GITC TNA protocol 6.4. Clicking the “forum” link for protocol 6.4 leads to this forum (but I did notice the forum itself says it’s for 6.3). Sorry for confusion there.

Getting more aggressive with bead washing did help. Before I was just tumbling and flicking by hand. In…Read more

]]> 450994c5a2591f6e214a1d6fb1772d9e Mike Axtell replied to the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/#post-9445 Wed, 08 Jul 2020 16:57:41 +1200 Thanks Phil, I appreciate your advice. I tried a series of extractions holding tissue amount constant (~ 18mg Arabidopsis leaves) and increasing silica bead usage over a ten-fold range. More beads indeed reduced clumping. However, the strong GITC contamination in the eluted TNA was not affected at all by the bead amounts.

I also did the same…Read more

]]> 5292fe765d9d972077c0e451ca7f48c0 Mike Axtell replied to the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/#post-9442 Thu, 02 Jul 2020 22:39:56 +1200 Hi,

I’m seeing strong absorbance at ~ 220-230 nm with TNA from this protocol (6.4). Suspect it is carryover of GITC. Also noticing that my silica beads tend to clump a little during binding, and only seem to “de-clump” upon elution. Wondering if the two observations (Guanidinium carryover and bead clumping) are connected, but even if unconnected,…Read more

]]> ed26a310e553c56606a3a24830b5a853 Mike Axtell replied to the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/#post-2116 Mon, 21 Jan 2019 22:15:42 +1200 Has anyone examined the recovery of microRNAs (~21 nt RNAs) using this TNA protocol, or with any of the BOMB protocols?

]]> 63110c121b819ac13874e9c10eebbc7b Mike Axtell became a registered member https://bomb.bio/activity/p/197/ Mon, 21 Jan 2019 22:13:47 +1200 0