bomb.bio | PStepper | Activity https://bomb.bio/members/pstepper/activity/ Activity feed for PStepper. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 745a08fac39415fcda4149aed9d1796b PStepper replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-8761 Sun, 14 Jul 2019 18:52:15 +1200 Hi Michael,

As you probably know it’s important to find the right balance between cytosine conversion, protection of methylated cytosines, and keeping DNA fragmentation to a minimum. But if your conversion efficiencies are lower, you’re right that I’d expect that either increasing the conversion temperature or incubation time (or even both) would…Read more

]]> 955f9a6913880ac3f2e18c1662c72afc PStepper replied to the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/#post-1615 Tue, 21 Aug 2018 15:22:55 +1200 reserved space

]]> 4f6def049a45f9643db58d668c73c891 PStepper started the topic BOMB protocol #1.1 magnetic core nanoparticles synthesis in the forum Core particles https://bomb.bio/forums/topic/bomb-protocol-1-1-magnetic-core-nanoparticles-synthesis/ Tue, 21 Aug 2018 15:15:55 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #1.1 magnetic core nanoparticles synthesis.
Here you can post all your questions and feedback!

*UPDATE*

Version 1.1

Version 1.0 of this protocol contained a typo on Page 3 (Step3) saying that 35% Ammonia solution is to be used, this should read 25% Ammonia solution and was…Read more

]]> 207fb873b71d835b0ecddf52c2b8e6f7 PStepper replied to the topic BOMB protocol #7.1 gDNA extraction in the forum gDNA https://bomb.bio/forums/topic/bomb-protocol-7-1-gdna-extraction/#post-1611 Tue, 21 Aug 2018 15:07:32 +1200 reserved space

]]> 628803d02e55797b0aca41e14c96020f PStepper started the topic BOMB protocol #7.1 gDNA extraction in the forum gDNA https://bomb.bio/forums/topic/bomb-protocol-7-1-gdna-extraction/ Tue, 21 Aug 2018 15:07:18 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #7.1 gDNA extraction.
Here you can post all your questions and feedback!

]]> 6a42fbcd9ef69da284f829bf5d2e832c PStepper replied to the topic BOMB protocol #8.2 RNA extraction from mammalian cells using GITC lysis in the forum RNA https://bomb.bio/forums/topic/bomb-protocol-8-2-rna-extraction-from-mammalian-cells-using-gitc-lysis/#post-1607 Tue, 21 Aug 2018 15:03:44 +1200 reserved space

]]> 33d0ca6f3bec7368efa2a888f177acfc PStepper started the topic BOMB protocol #8.2 RNA extraction from mammalian cells using GITC lysis in the forum RNA https://bomb.bio/forums/topic/bomb-protocol-8-2-rna-extraction-from-mammalian-cells-using-gitc-lysis/ Tue, 21 Aug 2018 15:03:28 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #8.2 RNA extraction from mammalian cells using GITC lysis.
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]]> a780cd966ea1c7b3d31b531d51a4fcb7 PStepper replied to the topic BOMB protocol #8.1 RNA extraction from mammalian cells using TRI reagent in the forum RNA https://bomb.bio/forums/topic/bomb-protocol-8-1-rna-extraction-from-mammalian-cells-using-tri-reagent/#post-1603 Tue, 21 Aug 2018 15:02:12 +1200 reserved space

]]> 22bff027064ac766a04089c737447e4d PStepper started the topic BOMB protocol #8.1 RNA extraction from mammalian cells using TRI reagent in the forum RNA https://bomb.bio/forums/topic/bomb-protocol-8-1-rna-extraction-from-mammalian-cells-using-tri-reagent/ Tue, 21 Aug 2018 14:59:55 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #8.1 RNA extraction from mammalian cells using TRI reagent.
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]]> 334c03f4493b23d80c2bbbf22fd7c2f1 PStepper started the topic BOMB protocol #9.1 Bisulfite conversion in the forum Bisulfite conversion https://bomb.bio/forums/topic/bomb-protocol-9-1-bisulfite-conversion/ Tue, 21 Aug 2018 14:19:04 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #9.1 Bisulfite conversion.
Here you can post all your questions and feedback!

]]> ac6482e9fbbe6a341de891ce459e9717 PStepper replied to the topic BOMB protocol #6.6 TNA extraction from E. coli in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-6-tna-extraction-from-e-coli/#post-1595 Tue, 21 Aug 2018 14:14:06 +1200 reserved space

]]> d46e3247fe6a68a2001a3d72794536f8 PStepper replied to the topic BOMB protocol #6.7 TNA extraction from environmental samples in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-7-tna-extraction-from-environmental-samples/#post-1594 Tue, 21 Aug 2018 14:13:49 +1200 reserved space

]]> b37ae01d4758d859ae2818fbd2a0a4c1 PStepper started the topic BOMB protocol #6.7 TNA extraction from environmental samples in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-7-tna-extraction-from-environmental-samples/ Tue, 21 Aug 2018 14:13:38 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.7 TNA extraction from environmental samples.
Here you can post all your questions and feedback!

]]> 198c934e4347600d5b48fd88c98e9b0d PStepper started the topic BOMB protocol #6.6 TNA extraction from E. coli in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-6-tna-extraction-from-e-coli/ Tue, 21 Aug 2018 14:12:27 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.6 TNA extraction from E. coli.
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]]> a0937a4708521f112813910519c8fcce PStepper replied to the topic BOMB protocol #6.5 TNA extraction from yeast in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-5-tna-extraction-from-yeast/#post-1591 Tue, 21 Aug 2018 14:10:44 +1200 reserved space

]]> 903e076ef2e097f51fda18171edf4bc0 PStepper started the topic BOMB protocol #6.5 TNA extraction from yeast in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-5-tna-extraction-from-yeast/ Tue, 21 Aug 2018 14:10:30 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.5 TNA extraction from yeast.
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]]> b3aece306526266c7e286d02df9f455f PStepper replied to the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/#post-1589 Tue, 21 Aug 2018 14:09:12 +1200 reserved space

]]> 0ca1b71ef07415e57d207a0d4e149ea5 PStepper started the topic BOMB protocol #6.4 TNA extraction from plants in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-4-tna-extraction-from-plants/ Tue, 21 Aug 2018 14:09:01 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.4 TNA extraction from plants.
Here you can post all your questions and feedback!

]]> 01da3defd2714f2c9ee1a0d52d031551 PStepper replied to the topic BOMB protocol #6.3 TNA extraction from tissue in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-3-tna-extraction-from-tissues/#post-1587 Tue, 21 Aug 2018 14:08:04 +1200 reserved space

]]> f449651f569920722d69c1acdc501f9c PStepper started the topic BOMB protocol #6.3 TNA extraction from tissue in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-3-tna-extraction-from-tissues/ Tue, 21 Aug 2018 14:07:50 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.3 TNA extraction from tissues.
Here you can post all your questions and feedback!

]]> 773d62856a4d7a298616008253efc643 PStepper replied to the topic BOMB protocol #6.2 TNA extraction from mammalian cells using TRI reagent in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-2-tna-extraction-from-mammalian-cells-using-tri-reagent/#post-1585 Tue, 21 Aug 2018 14:06:35 +1200 reserved space

]]> 8e845ad43a19cb34000d320f2e71d1fb PStepper started the topic BOMB protocol #6.2 TNA extraction from mammalian cells using TRI reagent in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-2-tna-extraction-from-mammalian-cells-using-tri-reagent/ Tue, 21 Aug 2018 14:06:23 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.2 TNA extraction from mammalian cells using TRI reagent.
Here you can post all your questions and feedback!

]]> beb53cecb049c57b064867bcb83e7f09 PStepper replied to the topic BOMB protocol #6.1 TNA extraction from mammalian cells using GITC lysis in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-1-tna-extraction-from-mammalian-cells-using-gitc-lysis/#post-1583 Tue, 21 Aug 2018 14:04:36 +1200 reserved space

]]> d094b2e323a37f1801a42a653801c55d PStepper started the topic BOMB protocol #6.1 TNA extraction from mammalian cells using GITC lysis in the forum TNA https://bomb.bio/forums/topic/bomb-protocol-6-1-tna-extraction-from-mammalian-cells-using-gitc-lysis/ Tue, 21 Aug 2018 14:03:49 +1200 Hi everyone,

this is the main discussion space for BOMB protocol #6.1 TNA extraction from mammalian cells using GITC lysis.
Here you can post all your questions and feedback!

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