bomb.bio | Rob Page | Activity https://bomb.bio/members/robertpage/activity/ Activity feed for Rob Page. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 9d8622a1e160f465f06554d1fa24567d Rob Page replied to the topic Implementing BOMB protocol for RNA extraction in Colombia in the forum COVID-19 https://bomb.bio/forums/topic/implementing-bomb-protocol-for-rna-extraction-in-colombia/#post-9396 Wed, 20 May 2020 20:01:12 +1200 Thank you very much for your reply Alejandro. It’s interesting that Proteinase K 10 mins @ 60 degrees should make a difference to the RdRP CT and not the RNaseP. Do you think it is simply breaking down the mucus/proteins to make the RNA more accessible to the beads? I will give it a go with our protocols tomorrow and let you (and everyone else…Read more

]]> 811c80c7299f56f443feafa809c11c7d Rob Page replied to the topic Implementing BOMB protocol for RNA extraction in Colombia in the forum COVID-19 https://bomb.bio/forums/topic/implementing-bomb-protocol-for-rna-extraction-in-colombia/#post-9394 Wed, 20 May 2020 14:53:20 +1200 Hi Alejandro,

I am working at King’s College London and Guy’s Hosptial to set up and validate as many RNA extraction protocols for SARS-CoV-2 as possible, including several silica based methods we have developed in house. We are seeing the same thing as you described. When compared to commercial kits, our RNaseP CTs are exactly the same, but we…Read more

]]> c800e5cbd0cf900987f573f3c96f5ce2 Rob Page replied to the topic in the forum Hillbilly Bead Viral RNA Extraction Protocol - BOMB Community https://bomb.bio/forums/reply/8992/#post-8995 Wed, 01 Apr 2020 11:58:40 +1200 Thank you so much for sharing your protocol and I’m glad to hear it worked with the spiked in RNA control. Similar to you, we have access to samples that have already been tested with commercial kits in diagnostic labs. We will compare and feedback here how it goes.

For the inactivation, we cannot propagate the virus in our lab but have a…Read more

]]> 871e711af08ebe5320131caf96cb2c97 Rob Page replied to the topic in the forum Adjusting BOMB protocol # 8.2 for SARS-CoV-2 extraction https://bomb.bio/forums/reply/8989/#post-8991 Tue, 31 Mar 2020 15:23:02 +1200 Hi,

I am also working on COVID19 diagnostics, based in London. We are going to try to use the GITC lysis RNA extraction protocol on our samples because sourcing commercial kits is starting to become a serious issue. If we can make our own then hopefully that would be much better!

I wondered what volume of lysis buffer you would recommend adding…Read more

]]> 6f5ea09bc232ca6fe155d9c3ab05c316 Rob Page became a registered member https://bomb.bio/activity/p/4477/ Tue, 31 Mar 2020 15:09:22 +1200 0