bomb.bio | Sheila Ons | Activity https://bomb.bio/members/sheilaons/activity/ Activity feed for Sheila Ons. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 aeebd4f8c171b1ebccc92b3df84ba8e6 Sheila Ons replied to the topic Implementing BOMB protocol for RNA extraction in Colombia in the forum COVID-19 https://bomb.bio/forums/topic/implementing-bomb-protocol-for-rna-extraction-in-colombia/#post-9492 Sat, 18 Jul 2020 19:21:01 +1200 Hi,

We are doing viral extraction by replacing GITC buffer by Trizol. Does we need to add RNA binding buffer to the samples? or can we follow the same protocol? What is RNA binding buffer used for?

Thanks a lot for your helpful answer.

 

Sheila

]]> 1757e7b944c0bac053c540263b68d348 Sheila Ons started the topic buffer for particle resuspenssion in the forum COVID-19 https://bomb.bio/forums/topic/buffer-for-particle-resuspenssion/ Fri, 26 Jun 2020 13:03:55 +1200 Hello, thanks again for your help!  For the SARS-COV2 protocol that you published here, which should be the better buffer to resuspend the silica beads? water? TE? PEG binding buffer? Thanks again for your answer!!

 

Sheila Ons.

Centro Regiona de Estudios Genómicos. UNLP. Buenos Aires. Argentina.

]]> 7befb44e20b79b3775a251ea00736c7f Sheila Ons replied to the topic SARS-CoV-2 Purification (Viral Transfer Media) - BOMB extraction protocol in the forum COVID-19 https://bomb.bio/forums/topic/the-official-sars-cov-2-bomb-extraction-protocol/page/2/#post-9438 Thu, 25 Jun 2020 14:27:07 +1200 Thanks Phil! I will let you know how it works once we tried.

Cheers.

 

Sheila

]]> 542d42319b301648807a4eaff0c39f64 Sheila Ons replied to the topic SARS-CoV-2 Purification (Viral Transfer Media) - BOMB extraction protocol in the forum COVID-19 https://bomb.bio/forums/topic/the-official-sars-cov-2-bomb-extraction-protocol/page/2/#post-9436 Thu, 25 Jun 2020 14:14:31 +1200 Hello, thanks for providing this protocol. If I use silica-coated beads, is there some step on this protocol that I must modify, or take extra-care? Or is exactly the same protocol for both kind of beads? Thank you in advance for your answer.

 

Sheila Ons

]]> e4190e93aa1f2068295b6d8c3e1d698a Sheila Ons became a registered member https://bomb.bio/activity/p/4742/ Mon, 22 Jun 2020 15:18:32 +1200 0