#7 DNA extraction

Hi Karina,

sorry for the late answer! Everything’s a bit crazy right now ^^

1) we never checked this, but I would assume it works with gram+ bacteria too. If not, one could think about digesting the cell wall before the lysis. We did something similar with yeast (#6.5).

2) Both bead-coatings should work with that protocol, although I personally prefer the silica-coated ones. I never tried these commercial beads, but don’t see why they wouldn’t work. However, other people had a few issues using commercial carboxyl-beads with our buffer systems. I would just give it a try or do a quick synthesis of BOMB-beads: https://bio-protocol.org/e3394

3) That depoends on the beads and what the supplier information says to do before using them. Our beads are simply suspended in water at a ratio of 1:1 wet mass volume to water volume.

4) I would again say, give it a try. We never tested this as far as I remember, but I don’t see why SDS shouldn’t work. Then again, I’m not a Chemist by training ^^

5) If you check in the original publication (https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000107) at Fig 2I our 260/280 ratios for gDNA are between 1.75 and 1.90, so yes, a bit higher is sometimes the case.

Hope it helps and sorry again for the delay. If you have any more questions, feel free to ask. I checked the notify button in this thread now.

Hi everyone!
Can I perform blood sample extractions with this protocol?
If someone tested it, they would like to know the sample volume used and relevant observations.  I intend to use this methodology to extract DNA from the swine blood virus.

Thank you in advance for your help.

Best regards,