Unfortunately it has grown rather quiet in the forum over the years. Yet countless numbers of Bots are continuing to try registering every day. And, since falling behind on security updates (captchas etc.), even by a few hours, often leads to scores successfully registering I have decided to suspend the registration for the forum. If you want to register, e-mail me.

Tim M, aka the Admin.

Bomb.bio has experienced an unprecedented number of spam bots registering over the last month.
Erasing all of them from the active user (forum contributor) list came at the risk of possibly deleting some real users. If this has happened to you, my apologies.
Cheers

Admin Tim

We are happy to host a new magnet rack designed by Quentin Gouil who adjusted the 3D printed “BOMB microtube rack” to fit Neodymium Block -12 mm x 5 mm x 2 mm | N52 Magnets. These specific magnets are available for purchase in Australia and the USA as well as some other nations from “AMF magnets” (not affiliated with BOMB.bio in any way).
The rack is intended to be 3D printed with the Magnets being glued in place.
The finished rack can hold up to 8x 1.5-2ml reaction tubes.

The .stl file can be found under
https://bomb.bio/protocols/   –> Magnetic Racks –> Printed racks

We have further refined our #2.1 MNP silica coating protocol, version 1.3 is now available.
Steps 1-4 have been changed. TEOS are being added later, leading to even better results.

Have you already been a big fan of #2.1_1.2 and cant wait to test version 1.3?
Or, maybe coating you very own Beads is a bit too much DIY but you love all our other protocols?
Let us and the science community out there know. We are on Facebook, twitter, Instagram, reddit and there is even a BOMB forum.

We discovered that protocol #3.1_BOMB_MNP_coating_carboxyl_groups_V1.0 was missing SDS in the list of required chemicals. It is however correctly mentioned in the description of the procedure on the nextpage.

Version 1.1 now contains the updated list of required chemicals.
No further changes were made.

Thank you to everyone who was already using and trusting our protocols and ideas while everything was still in pre-print. While we would not have chosen the fantastic bioRxiv.org option if we wouldn’t have been sure that the protocols provided are of the highest quality, we are happy to announce that the core BOMB publication is now fully peer reviewed and published in PLOS|Biology.

https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000107

We are happy to present the latest Nucleic Acid clean-up protocol “#4.4 BOMB clean-up and nucleotide removal using silica beads”. As always, you can find it on the protocols page or download it directly using this link.

Furthermore a minor modification has been made to “#4.1 BOMB clean-up and size exclusion using silica beads”. It now includes a two sentence description of the binding buffer creation process.