BOMB protocol #6.4 TNA extraction from plants

Viewing 4 posts - 16 through 19 (of 19 total)
  • Author
    Posts
  • #9483
    admin
    Keymaster

      Sorry for the wrong link. I fixed it and moved all of the plant posts to this thread. Can someone tell me if in case you have set email notifications they were automatically moved too?

      Cheers
      Tim M

      #9484
      Phil Oberacker
      Keymaster

        Thanks, Tim! 🙂

        #9486
        TimH
        Keymaster

          Hi Mike,

          Sorry we are slow to the conversation, however, I think Phil has covered off the points I would say are important. Paramount is more stringent washing of beads – I strongly suspect the 230 peak is GITC contamination; especially as you point out you see it even when there is no plant tissue at all (so this is a general bead issue, not just plant protocol). I would compare it to commercial gel extraction kits, which always produce a peak at 230, presumably because of GITC carryover in the column. I think the pertinent question is probably, what level of GITC carryover are you comfortable with? A little bit of carry over is not an issue (as in gel extraction), but too much and it can inhibit downstream enzymatic reactions. We have always managed to find a balance we are happy with.

          Cheers,

          TimH

           

          #9549
          Hary
          Participant

            Hi Mike,

            Did you finally resolve the peak problem at 220-230 nm and a low ratio of 260/280?. I have the same problem when testing protocol #6.4 for Eucalyptus species.

             

            Thanks

            Hary

          Viewing 4 posts - 16 through 19 (of 19 total)
          • You must be logged in to reply to this topic.