[tom]

Hi,

Im looking to use your protocol #4.2 with Sera-Mag Speedbead Carboxyl magnetic beads, however, the fragments I wish to isolate and purify are right on the lower cutoff for size, i.e., they are around 100 Bp in length, are there any known modifications to the protocol that can be made in order to allow for purifications of these fragments?

cheers

[admin]

I personally have always had to resort to agarose gel separation and extraction when trying to selectively purify at this small size.

Have a look at this link, I would say it sums up pretty much what is achievable using  SPRI :

https://ls.beckmancoulter.co.jp/files/appli_note/Gel_Free_Using_SPRIselect.pdf

(I am in no way affiliated with this company, have never purchased any of their products and can neither advocate for or against them. However, I do like their graphs.)

Cheers

Tim M

• This reply was modified 9 months ago by admin.

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