[adriano aguzzi]

We tried DYI magnetic nanoparticles a few years ago, and they always coalesced into large useless clumps of iron oxide. Did you observe this issue? If so, how did you resolve it?

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[admin]

Ahoy,

We have developed our protocol with as little clumping of MNPs as possible as one of the core goals. We achieve this by means of gradual precipitation, continuous stirring and minimal oxygen exposure. Have a look at the protocol which should answer your question in a more thorough manner:

https://bomb.bio/wp-content/uploads/2018/11/1.1_BOMB_MNP_synthesis_V1.2.pdf

If you have follow up questions afterwards or you would like to see what issues other users were facing with this protocol the best place to read and ask is probably here:

BOMB protocol #1.1 magnetic core nanoparticles synthesis

Thank you for your interest and happy extractions.
Tim M

[Phil Oberacker]

Hi Adriano,

as Tim already said, the key is to add the iron solution in a dropwise manner to the NaOH, while constantly stirring the latter. For this, we use a round-bottom flask with the NaOH in it and place it on a magnetic stirrer. We then put a dripping funnel on top in which we add the iron solution. You can find a thorough description of the setup either in the link Tim provided or here: https://bio-protocol.org/e3394

Cheers
Phil

[Author]

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