[Joshua_Horton]

Hey that seems like a really cool lab, I am looking to implement something similar. I suppose the easiest thing to do is just try skipping the isopropanol step. Now, one thing to note though is that a bunch of white stuff is kind of vague, could it be that there is so much genomic DNA it is precipitating to the point it is visualized? Part of the tricky thing here is even a little bacteria should produce WAY more than 50ng/uL of genomic DNA. How thick of a culture are you using. Another thing you could try is pelleting the precipitate or using a slightly different lysis method, I’ve tried garnet beads for 2 minutes on regular old votrexers and it works OK but not great.

 

Let me know if you ever figured it out!

[admin]

Ahoy,

I too have experienced significant issues when trying to isolate DNA from overly contaminant rich sources, massive amounts of white precipitate etc.

My guess was that this has to be some sort of water soluble proteins which will get bound to mag-beads once precipitated.  Could you try incorporating a proteinase K step?

 

Tim

[Author]

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