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For affinity purification of proteins it might be useful to have beads with a hydroxy group so it is possible to create a covalent bond with a peptide (bromcyanide technology). Would it be possible to adjust the coating protocol accordingly? I know there is also an option with a carboxy group but that does not work so well in our hands.
Dear Marco,
Thank you for your question. We did not try other coats than silica and carboxyl (MAA). However, instead of methacrylic acid you should be able to coat the core particles with hydroxyethyl methacrylate (HEMA) in the same kind of polymerisation reaction (simply to replace MAA with HEMA. This should provide a surface with OH groups.
Not sure if this is exactly what you need, but maybe worth a try.
Cheers,
T.
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