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Hi BOMB community
I’m working on the optimization of a bead-based size selection protocol. My aim is to enrich very short DNA fragments of 50-75bp, while depleting fragments of 150-175bp and above. I use silica beads and a binding buffer containing isopropanol and Guanidine thiocyanate.
I am able to enrich the small fragments, but I do get quite a large carry-over of the longer fragments in the size selected sample. I know that bead-based size selection does not really give a fixed cut-off, but I am very curious on whether it is possible to make the size selection more sharp/narrow, by manipulating some variables.
If anyone have some tips, ideas or knowledge that you are willing to share, it would be highly appreciated!
Kind regards, Anne
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