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- February 28, 2023 at 6:49 pm in reply to: BOMB protocol #4.2 Clean-up and size exclusion using carboxyl-coated MNPs #18249
Please in your protocol 2.1 you have mentioned in the first line:
<div>Combine your DNA sample with 5 µl stock solution of silica-coated magnetic</div>
<div>beads (BOMB protocol #2.1) and 2 volumes of binding buffer (for size exclusion</div>
<div>see Modification 1) in a 96-well PCR plate and mix until you receive a</div>
<div>homogenous suspension.</div>
<div>Question: What is the concentration of the silica coated magnetic stock solution ? also 2 volumes of binding buffer means 10 uL? Thank you</div>
<div>sorry for any inconvenience</div>
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