[Volker]

Thank you Tim for the quick reply!

I will check out Fuchsin as well and give feedback once I find something useful for the community.

Cheers,

Volker

[Volker]

Dear BOMB community,

first of all, let me say that I am a big fan of your work! We have just recently used the 3D models to print some magnetic racks and they work really well.

Now concerning the selfmade Tri reagent, also that seemed to work great. I just tested that reagent side-by-side with commercial reagents and yield as well as 260/280 ratios were almost identical.

My question aims at the Tri reagent itself: does anybody have any experience with using (organic) dyes to further color the organic phase (as in the original Trizol)? I know that the 8-hydroquinoline already gives this nice yellow color, but I would like to explore further options. Out of curiosity, I have tried Acridin Orange (mostly in organic phase, but aqueous phase looks somehow cloudy), Xylenecyanol and Direct Red (both dyes are in both phases). I will test some water-insoluble solvent dyes in the near future, but maybe somebody else already figured out which dyes work well.

Any feedback would be appreciated. Once again, thank you guys for the really great protocols and resources!

Cheers,

Volker

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