[Bernardo Pollak]

Hi, first of all thanks so much for all the amazing work you’ve done.

I have a question regarding the Speedbeads you suggest (45152105050250) vs what is suggested in the Openwetware protocol (65152105050250). Yours are hydrophilic, theirs are hydrophobic and both PEG/NaCl binding buffers are basically the same. I’ve tested hydrophilic vs hydrophobic Speedbeads together with Ampure XP beads in RNA purification and in enzymatic cleanup, and the hydrophilic beads clump in PEG buffer but the hydrophobic does not.

I know you protocols suggest binding RNA in guanidine buffers with isopropanol/ethanol but that seems like a bad idea for downstream NGS applications. Can you comment on this behavior, or suggest a way around of using the hydrophilic Speedbeads in RNA purification without guanidine buffers?

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