Thanks for your input and your great protocol. We have essentially arrived at what you outline in your protocol but are awaiting results (from actual patient samples). One thing that we have seen that matters is the use of carrier RNA. Our choice has been hybridoma cells that are available where we are (IMMY, immy.com), but we will need…Read more

Thanks for the link. I wondered how a lysis step that makes the viral RNA available for amplification would not be inactivating the same virus. I suspect that high titers are to blame. I had originally thought we would use a heated incubation of the lysis step until our colleagues got positive results without it. I think I am adding it…Read more