[Cor Breukel]

Thanks for the reply !

Best,

Cor

[Cor Breukel]

Hi,

Thanks for the provided protocols, I appreciate the effort ! I made my own beads and succesfully preped the first mini preps. I did use the buffers I had left over from a ROCHE kit since they are nearly the same.

 

I have a few questions:

In step 8 you state to mix first with ethanol for 5 minutes and than add the beads. I did  add ethanol and the beads together, and than mixed in a top over top rotor for 15 minutes (I work with 1.6 ml Eppendorf tubes). Is that ok ?

I noticed that after elution from the beads quite some volume is “taken up” by the beads: I used 75 µl Eb and got back 50 µl (I used 40 µl beads). This is as expected ?

 

Thanks,

 

Cor

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