We have been able to test this protocol on real samples and see it work, but with less efficiency compared to spin column extraction. We’d like to keep using this protocol for kit-less automation but looking for ways to increase extraction efficiency. Does anyone know if using silica beads vs. carboxylated beads will make a difference, I…Read more

We are about to try the new BOMB COVID-19 extraction, this is nearly identical to how we prepare for NGS and have good results, so I am very excited to get it working and automated (we’ll share the programming for the opentron system once it gets going). I do want to share though that we added 80mM of DTT to the lysis buffer with GTC…Read more

I am working with Bradley Stevenson and wanted to ask a few more questions. We are still using the above procedure with the Sera-Mag prepped beads without PEG. I saw another thread here about bead clumping (specifically carboxylated beads) that suggested it could be both a GTC issue and/or a nucleic acid concentration issue.

1. Your…Read more