[Jiyi]

Hi Michael,

Thanks for your reply, I repeat my experiment several time as keeping  beads on the magnet at the wash steps. The recovery raised to 20%, which is still low to me.

I am wondering that maybe the key problem is my beads.  I will update my progress if there are some good news.

Best,

Ji

 

[Jiyi]

hi everyone,

Is your recovery rate higher than 50%? My recovery is only 10% when I followed the protocol. Only difference is the silica-coated magnetic beads. I purchased the beads from other company. Its concentration is 50mg/ul and the size is 100nm.

The recovery of the EZ DNA methylation MagPrep kit(Zymo) is about 50-70%.

Best,

Ji

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