[MCGambetta]

It seems that the Nanodrop over-estimates the DNA concentration of our plasmid minipreps when we use our silica-coated beads. Does anyone have a suggestion? Many thanks

[MCGambetta]

Thanks for the tips Tomek, now it’s working.

[MCGambetta]

Right after synthesis, in alkali conditions, the magnetite pelleted on the magnet completely and quickly.

During the washes in H2O the magnetite pelleted more and more slowly, until it hardly pelleted anymore. The supernatant became progressively more brown in color.

We just ordered a better magnet.

Is it normal, though, that the magnetite pellets less efficiently or is this a sign of oxidation? We will use degassed H2O in any case next time (but would buffer be better?).

 

Thanks for the tips!

[MCGambetta]

Thanks for this awesome protocol 🙂

We got lovely magnetic beads after step 4 of the protocol…but the beads progressively became less and less magnetic during the MilliQ H2O washes.

I guess the culprit is our MilliQ water – its pH is 5   :-S

Along the lines of what R.B. suggested in reply #1942, could you suggest using buffer (which one?) instead of H2O for the washes?

Thanks in advance

[Author]

Posts