[Michael Dunnet]

Hi Jiyi,

I seem to get equivalent recovery rates with both BOMB and the EZ DNA methylation MagPrep kit. One thing that might be causing the issue is the washing steps. I know the zymo kit asks you to re-suspend the beads upon each wash; however, when I use the BOMB kit I keep the beads on the magnet and just add the ethanol to the tube for around 30s before removing it.

If that is something you already do I’m unsure on what the issue could be. Nevertheless, I hope this is helpful.

 

Cheers,

Michael

[Michael Dunnet]

Hey,

Just a quick question regarding the conversion step of the protocol. I’ve been getting lower conversion efficiencies compared to the previous kit I had been using (the EZ DNA methylation MagPrep kit by Zymo). I’ve noticed that the temperatures in the conversion step are much lower than in the Zymo kit despite everything else been very similar. I’m curious about the reasoning for this and whether or not you think I could improve the efficiency by increasing these temperatures.

Cheers,

Michael

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