[pazhanimuthu Annamlai]

Dear Admin,

It is really excellent protocol for a quick purification. It works better most cases but few samples showed one gene positive and one was negative.

Our elution buffer volume is 40 ul I wounder if we use more volume like 80 ul for eluting the RNA will it affect the detection.

I saw many automated machine are using 300 ul of VTM samples for isolation then final elution was at 150 ul then they are going 5 ul for RT-QPCR for the reaction volume of 20 ul

please give me an suggestion of sample volume to use in PCR reaction or any dilution if required. Since we are isolating total nucleic acid it might be inhibiting the reaction.

Thanks in advance!

Regards

Muthu

[Author]

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