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- July 28, 2020 at 9:17 pm in reply to: Implementing BOMB protocol for RNA extraction in Colombia #9497
Hi,
in Oxford UK we’ve set up the Covid BOMB technique on our Beckman FxP robot and we are following the Columbian protocol with proteinase K + heating the swab samples @ 60C in a 96 deepwell plate for 30 minutes. Not including the incubation times it can do 96 samples in about 40 minutes.
Problem is we’ve seen major clumping of the beads with some of the swab material, sometimes so bad the beads don’t pellet properly and are lost. We suspect this is due to DNA precipitating onto the beads so we are going to try adding DNAse I. Anyone seen this problem too?
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