bomb.bio | Timon | Activity https://bomb.bio/members/timon/activity/ Activity feed for Timon. Tue, 21 Apr 2026 20:24:12 +1200 https://buddypress.org/?v= en-US 30 hourly 2 093b7328e0837171944fe6551f5da666 Timon replied to the topic BOMB protocol #4.3 Gel extraction in the forum Gel extraction https://bomb.bio/forums/topic/4-1-bomb-gel-extraction/#post-9598 Wed, 10 Mar 2021 14:30:18 +1200 Is there any update on how to avoid these gel extraction issues? Thanks.

Cheers,

Timon

]]> 22dc21015855d90f060ee0125ad99ec4 Timon replied to the topic BOMB protocol #4.3 Gel extraction in the forum Gel extraction https://bomb.bio/forums/topic/4-1-bomb-gel-extraction/#post-9571 Sat, 30 Jan 2021 12:04:02 +1200 Hello Tim, thanks for you response,

I usually use 0.8% agarose gels, and try to dissolve 300mg of gel as a maximum for gel extraction. We tried to double the length of the gel dissolving step, but that did not prevent it. Neither did washing the carboxyl beads in advance, or heating the beadfilm in an attempt to dissolve remaining agarose.…Read more

]]> 8b15c84fb4f6679465432c8f9bc659b8 Timon replied to the topic BOMB protocol #4.3 Gel extraction in the forum Gel extraction https://bomb.bio/forums/topic/4-1-bomb-gel-extraction/#post-9563 Wed, 27 Jan 2021 14:14:49 +1200 Hey Everyone,

Our lab is really enjoying the beads, they work great. We have come across some issues when using them for gel extraction however. When we add isopropanol to the dissolved gel and the carboxylated beads we use, the beads tend to clump together. No vortexing, pipetting or even mechanical disruption tends to break the beads apart…Read more

]]> 0c8c32be54cb0026caf621c702c94db3 Timon became a registered member https://bomb.bio/activity/p/5223/ Wed, 27 Jan 2021 14:05:36 +1200 0