Ratio of magnetic core particles to reagents

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  • #9315
    admin
    Keymaster

      Hey David,

      My sincere apology for completely having missed your question. Considering how long ago you wrote it, I hope you have successfully silica coated MNPs in the meantime.

      Unfortunately I cannot answer your question concerning the odd ratio difference. To be honest looking at the protocol I am as befuddled by this as you are. However, I have forwarded the question to the part of the team who should be able to solve this mystery.

      What I can tell you is that the 22.5 grams are wet. This amount is pretty much exactly what I was able to harvest from the full scale MNP protocol.

      Best regards

      Tim M

       

       

      #9321
      Phil Oberacker
      Keymaster

        Hi David,

        you’re completely right! Thanks for pointing out the discrepancy! The correct ratio is the one given in the main protocol, not in the modifications paragraph. We published a more thorough version containing the correct ratios with bio-protocol (https://bio-protocol.org/e3394) and I just formated the protocol on the homepage accordingly. Sorry for the mislead!

        As Tim mentioned correctly, the 22.5 g wet mass are wet beads, they are not dried.

        I hope your synthesis worked anyway.
        Best regards
        Phil

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