- This topic has 21 replies, 11 voices, and was last updated July 11, 2021 at 7:19 pm by .
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The new protocols are uploaded 😀 thank you very much, @sebastian!
@svitak these might be interesting for you! Look for #5.3 and #5.4 (https://bomb.bio/protocols/)Awesome! Thanks @sebastian and @p-oberacker. I tried the protocol out earlier this week and it worked great!
Dear Sebastian,
Great to hear that you could adopt the protocols. BTW, you don’t need to synthesize the beads in anaerobic conditions. The synthesis protocol works well without argon or N2 environment.
It would be great if we could include midiprep or other protocols.
Cheers,
T.
Thanks for this great site.
I am trying to use this 5.1 protocol in my lab, and I find in the Materials , the Yeast Extract from BD Biosciences has a PN 212750 ( instead of 21270).
Just for your record.
Have a nice day
Ahoy,
Thanks Thomas you are absolutely correct.
I changed it and uploaded an updated version where I corrected the product number.
As all I did was add a single digit I figured it would be ok to just upload it under the same version number (V1.0).The change should take affect as soon as all caches are refreshed.
Cheers
TimHi
at first , thank for your helpful protocols.
I have question about exact concentration of M beads that used in plasmid extraction protocol
what is the concentration of beads in these protocols?
in 5.1 protocol you mention that use 50 micro liter of stock solution but in none of protocol mentioned what is the concentration of stock solution .
Thank You.
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