BOMB protocol #7.1 gDNA extraction

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  • #9722
    Emilly
    Participant

      Hi Tim, thanks for your answer!

      I will try tissue protocol then. Another question, I was reading some papers and they recommend pH 6 – 7 for silica DNA adsortion, could you explain why your pH lysis buffer is 7,6 – 8?

      Best wishes.

      #26288
      Mohammad Omran
      Participant

        Hello!

        we are planning to use your protocol for extracting DNA from Quinoa plants. We noticed that there is no step for Proteinase K.

        will adding this step will increase the qulaity of obtained DNA, any one has experience with this? when yes, how long is the incumbation time and how much one should use per sample and where in the protocol this should fit. Many thanks!

        Mohammad

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