Minipreps without centrifuge?

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  • #1940
    Keoni Gandall
    Participant

      Hey all,

      I’ve been looking for a magbead miniprep kit without doing any centrifugation step (in order to do full automation on an OpenTrons).  I found this – https://www.zymoresearch.com/zyppy , but am not really sure how the ‘Clearing Beads’ work. Do you guys have any tips on where to start investigating that?

      (Thanks for your patience, I am still a noob at this stuff)

      #1941
      TimH
      Keymaster

        Hi Keoni – do you mean clearing the beads as in putting them on a magnet? Not really sure how to help as not familiar with the Zymo kit…as a suggestion, you might like to try our BOMB protocols for mini-prep….Your timing is good – we just released the paper on BioRxiv https://www.biorxiv.org/content/early/2018/09/12/414516

        #9538
        weitinglin66
        Participant

          Wow! Just get a OpenTrons too! will definetly try the BOMB protocol!

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