Emilly

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  • March 21, 2022 at 8:57 am in reply to: BOMB protocol #7.1 gDNA extraction #9722
    Emilly
    Participant

      Hi Tim, thanks for your answer!

      I will try tissue protocol then. Another question, I was reading some papers and they recommend pH 6 – 7 for silica DNA adsortion, could you explain why your pH lysis buffer is 7,6 – 8?

      Best wishes.

      March 11, 2022 at 5:22 am in reply to: BOMB protocol #7.1 gDNA extraction #9712
      Emilly
      Participant

        Hello!

        I was reading your protocol of gDNA extraction with beads and a got confused with ratio mentioned 2:3:4. How is it used with blood sample and without TE buffer at beggining? It’s 140ul of blood and 20ul magnetic bead?

        Best regards.

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