[Jennifer Kuehl]

Hello

I am also getting very low 260/230 ratios.  I tried up to eight 80% ethanol washes, the ratios  improve with more washing but  they are still low ~.6 for gram positive bacteria and 1.7 for gram negative.  I am considering  a larger volumes of 70% ethanol (600ul) for the wash steps or perhaps a secondary bead clean-up.  Any recommendations?

[Author]

Posts