[Miriam Lenk]

Hi guys,

I have a very trivial question, sorry about that. I can’t seem to manage to seal my plates properly before vortexing during the washing steps. The 80% ethanol spills out of some of my wells, entering between the plate and the sealing foil. I tested two different types of foil, but both have the same problem, the plastic foil more and the aluminum foil less. I am pretty sure that I properly applied the foil because during PCR it seals perfectly. Maybe it’s because the ethonol does something to the glue?

Could you please tell me how you perform the washing step (vortexing or not) and what kind of foil or cover you use, if any? Do you use regular 0.2 ml PCR plates?

Thanks a lot in advance!

[Miriam Lenk]

Good to know… Thank you!

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