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- April 29, 2020 at 9:25 am in reply to: SARS-CoV-2 Purification (Viral Transfer Media) – BOMB extraction protocol #9370
Can you provide information about the chemical you are using with manufacturer & cat# (paramagnetic beads, GITC, Sarkosyl, EDTA etc.). I really want to get the similar result what you are getting with this BOMB protocol.
April 29, 2020 at 9:20 am in reply to: SARS-CoV-2 Purification (Viral Transfer Media) – BOMB extraction protocol #9369We have ordered Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophylic), 100 mL (Cat#45152105050350) from Cytiva ( https://www.cytivalifesciences.com/en/us). And the costomer service spoke with me regarding the purpose of the bead to use & the gave following suggestion-
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One point, This is not a popular bead. This one does have some sign of PCR inhibitor for some work, so is not one we would recommend. Especially for COVID19, we have no demand for this at all. Wondering why you pick this?
If you want better speed (speedbead double layer magnetic) than the regular bead (single layer), then may consider 6515 bead (so 100ml would be Cat#6515210505350).
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Please advise.
Thanks for the thermo info. But they don’t sell this item in Bangladesh. I am trying to get the rack from Alibaba & I found following products
Model#JH1719
Do you think N38 Neodymium(NdFeB) Magnet will work for our application? Please advise.
can I buy this 8 tube micro centrifuge from any commercial source? That will be really helpful if you can provide cat# & source.
April 25, 2020 at 3:58 am in reply to: SARS-CoV-2 Purification (Viral Transfer Media) – BOMB extraction protocol #9336I am wondering what type of Antifoam I should use in GITC Buffer preparation. There are different types of antifoam reagent & I am confused which one will be appropriate for this application.
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