Sarah

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  • in reply to: BOMB protocol #5.1 plasmid DNA extraction from E.coli #8844
    Sarah
    Participant

      Awesome! Thanks @sebastian and @p-oberacker. I tried the protocol out earlier this week and it worked great!

      in reply to: BOMB protocol #5.1 plasmid DNA extraction from E.coli #8786
      Sarah
      Participant

        Thanks so much Phil and Tim! That was very helpful! I appreciate it!

        in reply to: BOMB protocol #5.1 plasmid DNA extraction from E.coli #8775
        Sarah
        Participant

          Hey Bomb Beads Team!

          First and foremost, thanks for this amazing resource!

          Second and secondmost. Quick question. Would this and/or 5.2 protocol work with sermag beads? I gave it a shot and did not get very good results. But I just now realized that you say it was optimized for the silica beads and am wondering if it won’t work with other beads.

          Also, I was wondering why you use ethanol in your binding step? I’m used to using beads for DNA size selections and was under the impression based on that protocol that using so much ethanol would inhibit binding?

          Thanks!
          Sarah

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