[Timon]

Is there any update on how to avoid these gel extraction issues? Thanks.

Cheers,

Timon

[Timon]

Hello Tim, thanks for you response,

I usually use 0.8% agarose gels, and try to dissolve 300mg of gel as a maximum for gel extraction. We tried to double the length of the gel dissolving step, but that did not prevent it. Neither did washing the carboxyl beads in advance, or heating the beadfilm in an attempt to dissolve remaining agarose. Hopefully your colleague has a solution!

Cheers,

Timon

 

[Timon]

Hey Everyone,

Our lab is really enjoying the beads, they work great. We have come across some issues when using them for gel extraction however. When we add isopropanol to the dissolved gel and the carboxylated beads we use, the beads tend to clump together. No vortexing, pipetting or even mechanical disruption tends to break the beads apart again at this point. The yield is next to zero because of this. Does anyone have experience with these issues?

Thanks,

Timon

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