@pstepper
Forum Replies Created
- Posts
Hi Michael,
As you probably know it’s important to find the right balance between cytosine conversion, protection of methylated cytosines, and keeping DNA fragmentation to a minimum. But if your conversion efficiencies are lower, you’re right that I’d expect that either increasing the conversion temperature or incubation time (or even both) would boost the conversion rate. Maybe just give it a try with the 64 °C incubation for your application and report back the results to the community? And I’d advise to keep an eye out for DNA integrity and 5mC conversion (if you want to test this).
Maybe also have a look at these papers:
https://academic.oup.com/nar/article/36/22/e150/1195141#20603058
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093933
Best
Peter
